il 1β receptor antagonists  (Kingfisher Biotech)

Journal: Stem Cell Research & Therapy

Article Title: Obesity modulates hematopoietic stem cell fate decision via IL-1β induced p38/MAPK signaling pathway

doi: 10.1186/s13287-024-03915-w

Figure Lengend Snippet: Inhibition of IL-1β Changes in parameters caused by obesity. ( A ) Experimental scheme; ( B ) Statistics of the proportion of LSK cells in bone marrow mononuclear cells; ( C ) Proportion of LSK cell subsets; ( D ) Lineage differentiation of hematopoietic stem progenitor cells; ( E ) Expression level of genes in p38/MAPK pathway after anakinra treatment. (data = mean ± standard error, * represents a significant difference compared with ND group, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns represents no significant difference between the two groups)

Article Snippet: To interrupt IL-1β signaling, a human IL-1β receptor antagonist, anakinra (SOBI, #AB666-5823407), was intraperitoneally injected at a dosage of 10mg/kg following four weeks of the HFD [ ].

Techniques: Inhibition, Expressing

Journal: International Journal of Molecular Sciences

Article Title: HIV gp120 Protein Increases the Function of Connexin 43 Hemichannels and Pannexin-1 Channels in Astrocytes: Repercussions on Astroglial Function

doi: 10.3390/ijms21072503

Figure Lengend Snippet: The gp120-induced Cx43 hemichannel and Panx1 channel function depends on IL-1β/TNF-α signaling and activation of p38 MAPK/iNOS/P2X 7 /P2Y 1 /[Ca 2+ ] i -dependent pathways. Etd uptake rate normalized to control (dashed line) by astrocytes stimulated for 24 h with 10 ng/mL gp120 alone or plus the following agents: 100 ng/mL of IL-1ra+100 ng/mL of sTNF-αR1, 1 µM SB203580, 1 µM L-N6, 10 µM BAPTA, 1 µM MRS2179; 200 µM oxidized ATP (oATP) or 200 nM A740003. ** p < 0.01, gp120 vs. control; # p < 0.05, ## p < 0.01; pharmacological agents vs. gp120. Data were obtained from at least three independent experiments (see scatter dot plot) with three or more repeats each one (≥ 30 cells analyzed for each repeat).

Article Snippet: A soluble form of the TNF-α receptor (sTNF-αR1) and a recombinant receptor antagonist for IL-1β (IL-1ra) were from R&D Systems (Minneapolis, MN, USA).

Techniques: Activation Assay

Journal: International Journal of Molecular Sciences

Article Title: HIV gp120 Protein Increases the Function of Connexin 43 Hemichannels and Pannexin-1 Channels in Astrocytes: Repercussions on Astroglial Function

doi: 10.3390/ijms21072503

Figure Lengend Snippet: gp120 increases the release of IL-1β/TNF-α and the production of NO in astrocytes: involvement of Cx43 hemichannels and Panx1 channels. ( A , B ) Amounts of IL-1β ( A ) and TNF-α ( B ) found in the extracellular media of astrocytes cultured under control conditions (white bars) or stimulated for 24 h with 10 ng/mL gp120 alone (blue bars) or plus the following agents: 100 µM gap19 or 100 µM 10 panx1. * p < 0.001, gp120 vs. control. Data were obtained from at least three independent experiments (see scatter dot plot) with three or more repeats each one. ( C ) DAF-FM fluorescence normalized to control (dashed line) by astrocytes stimulated for 24 h with 10 ng/mL gp120 alone or plus the following agents: 100 µM gap19 or 100 µM 10 panx1. * p < 0.01, gp120 treatment vs. control, # p < 0.05, pharmacological agents vs. gp120. Data were obtained from at least three independent experiments (see scatter dot plot) with three or more repeats each one (≥ 35 cells analyzed for each repeat). ( D – G ) Fluorescence micrographs of basal NO production (DAF-FM, green) by astrocytes under control conditions ( D ) or stimulated for 24 h with 10 ng/mL gp120 alone ( E ) or plus 100 µM gap19 ( F ) or 100 µM 10 panx1 ( G ). Calibration bars: white = 80 µm.

Article Snippet: A soluble form of the TNF-α receptor (sTNF-αR1) and a recombinant receptor antagonist for IL-1β (IL-1ra) were from R&D Systems (Minneapolis, MN, USA).

Techniques: Cell Culture, Fluorescence